Unknown

Dataset Information

0

Archaea catalyze iron-dependent anaerobic oxidation of methane.


ABSTRACT: Anaerobic oxidation of methane (AOM) is crucial for controlling the emission of this potent greenhouse gas to the atmosphere. Nitrite-, nitrate-, and sulfate-dependent methane oxidation is well-documented, but AOM coupled to the reduction of oxidized metals has so far been demonstrated only in environmental samples. Here, using a freshwater enrichment culture, we show that archaea of the order Methanosarcinales, related to "Candidatus Methanoperedens nitroreducens," couple the reduction of environmentally relevant forms of Fe3+ and Mn4+ to the oxidation of methane. We obtained an enrichment culture of these archaea under anaerobic, nitrate-reducing conditions with a continuous supply of methane. Via batch incubations using [13C]methane, we demonstrated that soluble ferric iron (Fe3+, as Fe-citrate) and nanoparticulate forms of Fe3+ and Mn4+ supported methane-oxidizing activity. CO2 and ferrous iron (Fe2+) were produced in stoichiometric amounts. Our study connects the previous finding of iron-dependent AOM to microorganisms detected in numerous habitats worldwide. Consequently, it enables a better understanding of the interaction between the biogeochemical cycles of iron and methane.

SUBMITTER: Ettwig KF 

PROVIDER: S-EPMC5111651 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Archaea catalyze iron-dependent anaerobic oxidation of methane.

Ettwig Katharina F KF   Zhu Baoli B   Speth Daan D   Keltjens Jan T JT   Jetten Mike S M MSM   Kartal Boran B  

Proceedings of the National Academy of Sciences of the United States of America 20161024 45


Anaerobic oxidation of methane (AOM) is crucial for controlling the emission of this potent greenhouse gas to the atmosphere. Nitrite-, nitrate-, and sulfate-dependent methane oxidation is well-documented, but AOM coupled to the reduction of oxidized metals has so far been demonstrated only in environmental samples. Here, using a freshwater enrichment culture, we show that archaea of the order <i>Methanosarcinales</i>, related to "<i>Candidatus</i> Methanoperedens nitroreducens," couple the redu  ...[more]

Similar Datasets

| S-EPMC4683180 | biostudies-literature
| S-EPMC7810693 | biostudies-literature
| S-EPMC7360803 | biostudies-literature
| S-EPMC4209987 | biostudies-literature
| S-EPMC3187098 | biostudies-literature
| S-EPMC194967 | biostudies-literature
| S-EPMC10923174 | biostudies-literature
| S-EPMC3697569 | biostudies-literature
| S-EPMC9539880 | biostudies-literature
| S-EPMC3970540 | biostudies-literature