Dataset Information

Complete Genome Sequence of the Siphoviral Bacteriophage Ec-ZZ2, Which Is Capable of Lysing Enterococcus faecium.

ABSTRACT: A virulent bacteriophage (Ec-ZZ2) that infects Enterococcus faecium was isolated from sewage. The bacteriophage belongs to the family Siphoviridae and has a linear double-stranded DNA genome, with a length of 41,170 bp and a 34.59% G+C content, which is highly similar to Enterococcus phage IME-EF4.

SUBMITTER: Li J

PROVIDER: S-EPMC5114367 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Publications

Complete Genome Sequence of the Siphoviral Bacteriophage Ec-ZZ2, Which Is Capable of Lysing Enterococcus faecium.

Li Jinghua J Shi Hongyan H Zhao Chunyan C Hao Yuchong Y He Yingze Y Sun Yanbo Y

Genome announcements 20161117 6

A virulent bacteriophage (Ec-ZZ2) that infects Enterococcus faecium was isolated from sewage. The bacteriophage belongs to the family Siphoviridae and has a linear double-stranded DNA genome, with a length of 41,170 bp and a 34.59% G+C content, which is highly similar to Enterococcus phage IME-EF4. ...[more]

PMID: 27856575

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Project description:Enterococci are among the leading causes of hospital-acquired infections in the United States and Europe, with Enterococcus faecalis and Enterococcus faecium being the two most common species isolated from enterococcal infections. In the last decade, the proportion of enterococcal infections caused by E. faecium has steadily increased compared to other Enterococcus species. Although the underlying mechanism for the gradual replacement of E. faecalis by E. faecium in the hospital environment is not yet understood, many studies using genotyping and phylogenetic analysis have shown the emergence of a globally dispersed polyclonal subcluster of E. faecium strains in clinical environments. Systematic study of the molecular epidemiology and pathogenesis of E. faecium has been hindered by the lack of closed, complete E. faecium genomes that can be used as references.In this study, we report the complete genome sequence of the E. faecium strain TX16, also known as DO, which belongs to multilocus sequence type (ST) 18, and was the first E. faecium strain ever sequenced. Whole genome comparison of the TX16 genome with 21 E. faecium draft genomes confirmed that most clinical, outbreak, and hospital-associated (HA) strains (including STs 16, 17, 18, and 78), in addition to strains of non-hospital origin, group in the same clade (referred to as the HA clade) and are evolutionally considerably more closely related to each other by phylogenetic and gene content similarity analyses than to isolates in the community-associated (CA) clade with approximately a 3-4% average nucleotide sequence difference between the two clades at the core genome level. Our study also revealed that many genomic loci in the TX16 genome are unique to the HA clade. 380 ORFs in TX16 are HA-clade specific and antibiotic resistance genes are enriched in HA-clade strains. Mobile elements such as IS16 and transposons were also found almost exclusively in HA strains, as previously reported.Our findings along with other studies show that HA clonal lineages harbor specific genetic elements as well as sequence differences in the core genome which may confer selection advantages over the more heterogeneous CA E. faecium isolates. Which of these differences are important for the success of specific E. faecium lineages in the hospital environment remain(s) to be determined.

Dataset Information

Complete Genome Sequence of the Siphoviral Bacteriophage Ec-ZZ2, Which Is Capable of Lysing Enterococcus faecium.

Publications

Complete Genome Sequence of the Siphoviral Bacteriophage Ec-ZZ2, Which Is Capable of Lysing Enterococcus faecium.

Similar Datasets

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