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ALKBH1-Mediated tRNA Demethylation Regulates Translation.

ABSTRACT: tRNA is a central component of protein synthesis and the cell signaling network. One salient feature of tRNA is its heavily modified status, which can critically impact its function. Here, we show that mammalian ALKBH1 is a tRNA demethylase. It mediates the demethylation of N1-methyladenosine (m1A) in tRNAs. The ALKBH1-catalyzed demethylation of the target tRNAs results in attenuated translation initiation and decreased usage of tRNAs in protein synthesis. This process is dynamic and responds to glucose availability to affect translation. Our results uncover reversible methylation of tRNA as a new mechanism of post-transcriptional gene expression regulation.

SUBMITTER: Liu F

PROVIDER: S-EPMC5119773 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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ALKBH1-Mediated tRNA Demethylation Regulates Translation.

Liu Fange F Clark Wesley W Luo Guanzheng G Wang Xiaoyun X Fu Ye Y Wei Jiangbo J Wang Xiao X Hao Ziyang Z Dai Qing Q Zheng Guanqun G Ma Honghui H Han Dali D Evans Molly M Klungland Arne A Pan Tao T He Chuan C

Cell 20161013 3

tRNA is a central component of protein synthesis and the cell signaling network. One salient feature of tRNA is its heavily modified status, which can critically impact its function. Here, we show that mammalian ALKBH1 is a tRNA demethylase. It mediates the demethylation of N1-methyladenosine (m1A) in tRNAs. The ALKBH1-catalyzed demethylation of the target tRNAs results in attenuated translation initiation and decreased usage of tRNAs in protein synthesis. This process is d ...[more]

PMID: 27745969

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