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Native halo-tolerant plant growth promoting rhizobacteria Enterococcus and Pantoea sp. improve seed yield of Mungbean (Vigna radiata L.) under soil salinity by reducing sodium uptake and stress injury.

ABSTRACT: The beneficial microbial-plant interaction plays important role in the soil health, crop growth and productivity. Plant growth promoting rhizobacteria (PGPR) are such beneficial microorganisms, which in association with plant roots not only promote their growth but also help in counteracting the detrimental effects of soil stresses. Salt stress is one such stress, frequently confronted by the plants. The present study aimed at isolation and identification of PGPR inhabiting the mungbean rhizosphere, testing them for salt (NaCl) tolerance and subsequently in salt-supplemented mungbean crop. For this purpose, two salt-tolerant bacterial strains belonging to genus Pantoea and Enterococcus, characterized for their P-solubilization ability, indole acetic acid and siderophore production were selected. These two PGPR were further evaluated for their effect on the salt-stressed mungbean plants, grown at two salt concentrations (5 and 10 dS/m). The plants treated with the combination of PGPR showed better performance in growth (16-37 %) and yield (22-32 %), under salt stress, as compared with control. The increasing salt concentration was found to increase the membrane damage, Na+ concentration in the plants. PGPR treatments effectively reduced the Na+ concentration (17-41 %), membrane damage (1.1-1.5 folds) and enhanced the antioxidants i.e. ascorbic acid (8-26 %) and glutathione (10-30 %) in salt-stressed plants, in comparison to uninoculated stressed plants. Overall, the results indicated that both PGPR were effective as stress mitigators however, in combination they showed relatively better improvement in growth, yield as well as oxidative parameters of the salt-affected plants. These findings about the effects of native salt-tolerant PGPR Pantoea and Enterococcus sp. in mungbean crop are novel.

SUBMITTER: Panwar M

PROVIDER: S-EPMC5120033 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Native halo-tolerant plant growth promoting rhizobacteria Enterococcus and Pantoea sp. improve seed yield of Mungbean (Vigna radiata L.) under soil salinity by reducing sodium uptake and stress injury.

Panwar Meenu M Tewari Rupinder R Nayyar Harsh H

Physiology and molecular biology of plants : an international journal of functional plant biology 20161007 4

The beneficial microbial-plant interaction plays important role in the soil health, crop growth and productivity. Plant growth promoting rhizobacteria (PGPR) are such beneficial microorganisms, which in association with plant roots not only promote their growth but also help in counteracting the detrimental effects of soil stresses. Salt stress is one such stress, frequently confronted by the plants. The present study aimed at isolation and identification of PGPR inhabiting the mungbean rhizosph ...[more]

PMID: 27924118

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Project description:Soil salinity is one of the major limiting factors in plant growth regulation. Salinity-tolerant endophytic bacteria (STEB) can be used to alleviate the negative effects of salinity and promote plant growth. In this study, thirteen endophytic bacteria were isolated from mungbean roots and tested for NaCl salt-tolerance up to 4%. Six bacterial isolates, TMB2, TMB3, TMB5, TMB6, TMB7 and TMB9, demonstrated the ability to tolerate salt. Plant growth-promoting properties such as phosphate solubilization, indole-3-acetic acid (IAA) production, nitrogen fixation, zinc solubilization, biofilm formation and hydrolytic enzyme production were tested in vitro under saline conditions. Eight bacterial isolates indicated phosphate solubilization potential ranging from 5.8-17.7 μg mL-1, wherein TMB6 was found most efficient. Ten bacterial isolates exhibited IAA production ranging from 0.3-2.1 μg mL-1, where TMB7 indicated the highest potential. All the bacterial isolates except TMB13 exhibited nitrogenase activity. Three isolates, TMB6, TMB7 and TMB9, were able to solubilize zinc on tris-minimal media. All isolates were capable of forming biofilm except TMB12 and TMB13. Only TMB2, TMB6 and TMB7 exhibited cellulase activity, while TMB2 and TMB7 exhibited pectinase production. Based on in vitro testing, six efficient STEB were selected and subjected to the further studies. 16S rRNA gene sequencing of efficient STEB revealed the maximum similarity between TMB2 and Rhizobium pusense, TMB3 and Agrobacterium leguminum, TMB5 and Achromobacter denitrificans, TMB6 and Pseudomonas extremorientalis, TMB7 and Bradyrhizobium japonicum and TMB9 and Serratia quinivorans. This is the first international report on the existence of A. leguminum, A. denitrificans, P. extremorientalis and S. quinivorans inside the roots of mungbean. Under controlled-conditions, inoculation of P. extremorientalis TMB6, B. japonicum TMB7 and S. quinivorans TMB9 exhibited maximum potential to increase plant growth parameters; specifically plant dry weight was increased by up to 52%, 61% and 45%, respectively. Inoculation of B. japonicum TMB7 displayed the highest potential to increase plant proline, glycine betaine and total soluble proteins contents by 77%, 78% and 64%, respectively, compared to control under saline conditions. It is suggested that the efficient STEB could be used as biofertilizers for mungbean crop productivity under saline conditions after field-testing.

Dataset Information

Native halo-tolerant plant growth promoting rhizobacteria Enterococcus and Pantoea sp. improve seed yield of Mungbean (Vigna radiata L.) under soil salinity by reducing sodium uptake and stress injury.

Publications

Native halo-tolerant plant growth promoting rhizobacteria <i>Enterococcus</i> and <i>Pantoea</i> sp. improve seed yield of Mungbean (<i>Vigna radiata</i> L.) under soil salinity by reducing sodium uptake and stress injury.

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