Increased gene dosage for β- and κ-casein in transgenic cattle improves milk composition through complex effects.

ABSTRACT: We have previously generated transgenic cattle with additional copies of bovine β- and κ casein genes. An initial characterisation of milk produced with a hormonally induced lactation from these transgenic cows showed an altered milk composition with elevated β-casein levels and twofold increased κ-casein content. Here we report the first in-depth characterisation of the composition of the enriched casein milk that was produced through a natural lactation. We have analyzed milk from the high expressing transgenic line TG3 for milk composition at early, peak, mid and late lactation. The introduction of additional β- and κ-casein genes resulted in the expected expression of the transgene derived proteins and an associated reduction in the size of the casein micelles. Expression of the transg

SUBMITTER: Laible G 

PROVIDER: S-EPMC5120311 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature