Dataset Information

Micro-CT vs. Whole Body Multirow Detector CT for Analysing Bone Regeneration in an Animal Model.

ABSTRACT:

Objectives

Compared with multirow detector CT (MDCT), specimen (ex vivo) micro-CT (?CT) has a significantly higher (~ 30 x) spatial resolution and is considered the gold standard for assessing bone above the cellular level. However, it is expensive and time-consuming, and when applied in vivo, the radiation dose accumulates considerably. The aim of this study was to examine whether the lower resolution of the widely used MDCT is sufficient to qualitatively and quantitatively evaluate bone regeneration in rats.

Methods

Forty critical-size defects (5mm) were placed in the mandibular angle of rats and covered with coated bioactive titanium implants to promote bone healing. Five time points were selected (7, 14, 28, 56 and 112 days). ?CT and MDCT were used to evaluate the defect region to determine the bone volume (BV), tissue mineral density (TMD) and bone mineral content (BMC).

Results

MDCT constantly achieved higher BV values than ?CT (10.73±7.84 mm3 vs. 6.62±4.96 mm3, p<0.0001) and consistently lower TMD values (547.68±163.83 mm3 vs. 876.18±121.21 mm3, p<0.0001). No relevant difference was obtained for BMC (6.48±5.71 mm3 vs. 6.15±5.21 mm3, p = 0.40). BV and BMC showed very strong correlations between both methods, whereas TMD was only moderately correlated (r = 0.87, r = 0.90, r = 0.68, p < 0.0001).

Conclusions

Due to partial volume effects, MDCT overestimated BV and underestimated TMD but accurately determined BMC, even in small volumes, compared with ?CT. Therefore, if bone quantity is a sufficient end point, a considerable number of animals and costs can be saved, and compared with in vivo ?CT, the required dose of radiation can be reduced.

SUBMITTER: Bissinger O

PROVIDER: S-EPMC5120815 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Publications

Micro-CT vs. Whole Body Multirow Detector CT for Analysing Bone Regeneration in an Animal Model.

Bissinger Oliver O Kirschke Jan S JS Probst Florian Andreas FA Stauber Martin M Wolff Klaus-Dietrich KD Haller Bernhard B Götz Carolin C Plank Christian C Kolk Andreas A

PloS one 20161123 11

<h4>Objectives</h4>Compared with multirow detector CT (MDCT), specimen (ex vivo) micro-CT (μCT) has a significantly higher (~ 30 x) spatial resolution and is considered the gold standard for assessing bone above the cellular level. However, it is expensive and time-consuming, and when applied in vivo, the radiation dose accumulates considerably. The aim of this study was to examine whether the lower resolution of the widely used MDCT is sufficient to qualitatively and quantitatively evaluate bon ...[more]

PMID: 27880788

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Project description:The size of field-of-view (FOV) of a microcomputed tomography (CT) system can be increased by offsetting the detector. The increased FOV is beneficial in many applications. All prior investigations, however, have been focused to the case in which the increased FOV after offset-detector acquisition can cover the transaxial extent of an object fully. Here, the authors studied a new problem where the FOV of a micro-CT system, although increased after offset-detector acquisition, still covers an interior region-of-interest (ROI) within the object.An interior-ROI-oriented micro-CT scan with an offset detector poses a difficult reconstruction problem, which is caused by both detector offset and projection truncation. Using the projection completion techniques, the authors first extended three previous reconstruction methods from offset-detector micro-CT to offset-detector interior micro-CT. The authors then proposed a novel method which combines two of the extended methods using a frequency split technique. The authors tested the four methods with phantom simulations at 9.4%, 18.8%, 28.2%, and 37.6% detector offset. The authors also applied these methods to physical phantom datasets acquired at the same amounts of detector offset from a customized micro-CT system.When the detector offset was small, all reconstruction methods showed good image quality. At large detector offset, the three extended methods gave either visible shading artifacts or high deviation of pixel value, while the authors' proposed method demonstrated no visible artifacts and minimal deviation of pixel value in both the numerical simulations and physical experiments.For an interior micro-CT with an offset detector, the three extended reconstruction methods can perform well at a small detector offset but show strong artifacts at a large detector offset. When the detector offset is large, the authors' proposed reconstruction method can outperform the three extended reconstruction methods by suppressing artifacts and maintaining pixel values.

Dataset Information

Micro-CT vs. Whole Body Multirow Detector CT for Analysing Bone Regeneration in an Animal Model.

Objectives

Methods

Results

Conclusions

Publications

Micro-CT vs. Whole Body Multirow Detector CT for Analysing Bone Regeneration in an Animal Model.

Similar Datasets

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