Ontology highlight
ABSTRACT:
SUBMITTER: Su T
PROVIDER: S-EPMC5121644 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Su Tianyuan T Liu Fapeng F Gu Pengfei P Jin Haiying H Chang Yizhao Y Wang Qian Q Liang Quanfeng Q Qi Qingsheng Q
Scientific reports 20161124
Homologous recombination-mediated genome engineering has been broadly applied in prokaryotes with high efficiency and accuracy. However, this method is limited in realizing larger-scale genome editing with numerous genes or large DNA fragments because of the relatively complicated procedure for DNA editing template construction. Here, we describe a CRISPR-Cas9 assisted non-homologous end-joining (CA-NHEJ) strategy for the rapid and efficient inactivation of bacterial gene (s) in a homologous rec ...[more]