Irx4 Marks a Multipotent, Ventricular-Specific Progenitor Cell.
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ABSTRACT:
SUBMITTER: Nelson DO
PROVIDER: S-EPMC5123941 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Publications
Irx4 Marks a Multipotent, Ventricular-Specific Progenitor Cell.
Nelson Daryl O DO Lalit Pratik A PA Biermann Mitch M Markandeya Yogananda S YS Capes Deborah L DL Addesso Luke L Patel Gina G Han Tianxiao T John Manorama C MC Powers Patricia A PA Downs Karen M KM Kamp Timothy J TJ Lyons Gary E GE
Stem cells (Dayton, Ohio) 20160913 12
While much progress has been made in the resolution of the cellular hierarchy underlying cardiogenesis, our understanding of chamber-specific myocardium differentiation remains incomplete. To better understand ventricular myocardium differentiation, we targeted the ventricle-specific gene, Irx4, in mouse embryonic stem cells to generate a reporter cell line. Using an antibiotic-selection approach, we purified Irx4<sup>+</sup> cells in vitro from differentiating embryoid bodies. The isolated Irx4 ...[more]
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