Ontology highlight
ABSTRACT:
SUBMITTER: Tandonnet S
PROVIDER: S-EPMC5124356 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Tandonnet Sophie S Torres Tatiana Teixeira TT
Genomics data 20161118
One limitation of the widely used RNA-seq method is that long transcripts are represented by more reads than shorter transcripts, resulting in a biased estimation of expression levels. The 3' RNA-seq method, which yields only one sequence per transcript, bypasses this limitation. Here, RNA was extracted from two samples, in which we expected to find differentially expressed genes. Each was processed by both traditional and 3' RNA-seq protocols. Both methods yielded similar differentially express ...[more]