Identification of a key cholesterol binding enhancement motif in translocator protein 18 kDa.
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ABSTRACT:
SUBMITTER: Li F
PROVIDER: S-EPMC5125615 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
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Identification of a key cholesterol binding enhancement motif in translocator protein 18 kDa.
Li Fei F Liu Jian J Valls Lance L Hiser Carrie C Ferguson-Miller Shelagh S
Biochemistry 20150210 7
Translocator protein 18 kDa (TSPO) in the mitochondrial outer membrane has been implicated in cholesterol transport regulating steroidogenesis. A human single polymorphism associated with anxiety disorders (A147T) and reduced pregnenolone production is adjacent to TSPO's cholesterol binding motif. In a mutant mimicking this polymorphism, we observe a lower level of binding of cholesterol. Further, three residues preceding A147 are more hydrophilic in a bacterial TSPO that has an affinity for cho ...[more]
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