Dataset Information

Identification of Novel Equine (Equus caballus) Tendon Markers Using RNA Sequencing.

ABSTRACT: Although several tendon-selective genes exist, they are also expressed in other musculoskeletal tissues. As cell and tissue engineering is reliant on specific molecular markers to discriminate between cell types, tendon-specific genes need to be identified. In order to accomplish this, we have used RNA sequencing (RNA-seq) to compare gene expression between tendon, bone, cartilage and ligament from horses. We identified several tendon-selective gene markers, and established eyes absent homolog 2 (EYA2) and a G-protein regulated inducer of neurite outgrowth 3 (GPRIN3) as specific tendon markers using RT-qPCR. Equine tendon cells cultured as three-dimensional spheroids expressed significantly greater levels of EYA2 than GPRIN3, and stained positively for EYA2 using immunohistochemistry. EYA2 was also found in fibroblast-like cells within the tendon tissue matrix and in cells localized to the vascular endothelium. In summary, we have identified EYA2 and GPRIN3 as specific molecular markers of equine tendon as compared to bone, cartilage and ligament, and provide evidence for the use of EYA2 as an additional marker for tendon cells in vitro.

SUBMITTER: Kuemmerle JM

PROVIDER: S-EPMC5126783 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Identification of Novel Equine (Equus caballus) Tendon Markers Using RNA Sequencing.

Kuemmerle Jan M JM Theiss Felix F Okoniewski Michal J MJ Weber Fabienne A FA Hemmi Sonja S Mirsaidi Ali A Richards Peter J PJ Cinelli Paolo P

Genes 20161110 11

Although several tendon-selective genes exist, they are also expressed in other musculoskeletal tissues. As cell and tissue engineering is reliant on specific molecular markers to discriminate between cell types, tendon-specific genes need to be identified. In order to accomplish this, we have used RNA sequencing (RNA-seq) to compare gene expression between tendon, bone, cartilage and ligament from horses. We identified several tendon-selective gene markers, and established eyes absent homolog 2 ...[more]

PMID: 27834918

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Dataset Information

Identification of Novel Equine (Equus caballus) Tendon Markers Using RNA Sequencing.

Publications

Identification of Novel Equine (Equus caballus) Tendon Markers Using RNA Sequencing.

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