Dataset Information

The histone genes cluster in Rhynchosciara americana and its transcription profile in salivary glands during larval development.

ABSTRACT: In this work we report the characterization of the Rhynchosciara americana histone genes cluster nucleotide sequence. It spans 5,131 bp and contains the four core histones and the linker histone H1. Putative control elements were detected. We also determined the copy number of the tandem repeat unit through quantitative PCR, as well as the unequivocal chromosome location of this unique locus in chromosome A band 13. The data were compared with histone clusters from the genus Drosophila, which are the closest known homologues.

SUBMITTER: Siviero F

PROVIDER: S-EPMC5127150 | biostudies-literature | 2016 Oct-Dec

REPOSITORIES: biostudies-literature

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The histone genes cluster in Rhynchosciara americana and its transcription profile in salivary glands during larval development.

Siviero Fábio F Rezende-Teixeira Paula P Andrade Alexandre de A Santelli Roberto Vicente RV Machado-Santelli Glaucia Maria GM

Genetics and molecular biology 20161010 4

In this work we report the characterization of the Rhynchosciara americana histone genes cluster nucleotide sequence. It spans 5,131 bp and contains the four core histones and the linker histone H1. Putative control elements were detected. We also determined the copy number of the tandem repeat unit through quantitative PCR, as well as the unequivocal chromosome location of this unique locus in chromosome A band 13. The data were compared with histone clusters from the genus Drosophila, which ar ...[more]

PMID: 27727361

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Project description:When misexpressed in late Drosophila prepupae, the transcription factor Fork head (Fkh) blocks death of the larval salivary glands that normally occurs in the early pupa. The aim of the experiment was to identify genes responding to Fkh that might mediate the effect of the protein on cell death and other biological processes. Fkh was expressed in the line P[hs-Fkh111] from a heat-inducible transgene that encodes wild-type Fkh protein. Expression of Fkh was induced by incubating prepupae for 30 min in a 37 Â°C water bath, starting at 9.5 hours after puparium formation. Salivary glands were dissected at 14 hours after puparium formation and RNA isolated for microarray analysis with Affymetrix GeneChips. Control samples were obtained from w1118 animals treated the same way. The microarray analysis identified 55 genes annotated as functioning in apoptosis whose expression was at least 1.5-fold changed by Fkh. These genes include the death genes hid and reaper, which play a central role in the control of salivary gland death. Other groups of significantly enriched genes include genes functioning in autophagy, steroid-signaling pathways, salivary gland secretion, and phospholipid metabolism. In addition, the microarray data identify genes as responsive to Fkh that are known to be controlled by the FOXA counterparts of Fkh in vertebrates, indicating that target genes and biological processes controlled by Fkh are evolutionarily conserved. SUBMITTER_CITATION: Liu and Lehmann, 2008: Genes and biological processes controlled by the Drosophila FOXA orthologue Fork head. Insect Molecular Biology, 17, 91-101; link: http://www.blackwell-synergy.com/toc/imb/17/2) Experiment Overall Design: Experimental RNA samples were obtained in 3 biological replicates from fkh-expressing salivary glands (SG_Fkh_14APF_rep1, SG_Fkh_14APF_rep2, SG_Fkh_14APF_rep3) and compared to control samples obtained in two biological replicates from the salivary glands of w1118 control animals (SG_w1118(3)_14APF_rep1, SG_w1118(3)_14APF_rep2). All samples were compared using dChip and normalized to the same baseline array (median intensity 134).

Dataset Information

The histone genes cluster in Rhynchosciara americana and its transcription profile in salivary glands during larval development.

Publications

The histone genes cluster in Rhynchosciara americana and its transcription profile in salivary glands during larval development.

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