Ontology highlight
ABSTRACT:
SUBMITTER: Abudayyeh OO
PROVIDER: S-EPMC5127784 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Abudayyeh Omar O OO Gootenberg Jonathan S JS Konermann Silvana S Joung Julia J Slaymaker Ian M IM Cox David B T DB Shmakov Sergey S Makarova Kira S KS Semenova Ekaterina E Minakhin Leonid L Severinov Konstantin K Regev Aviv A Lander Eric S ES Koonin Eugene V EV Zhang Feng F
Science (New York, N.Y.) 20160602 6299
The clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated genes (Cas) adaptive immune system defends microbes against foreign genetic elements via DNA or RNA-DNA interference. We characterize the class 2 type VI CRISPR-Cas effector C2c2 and demonstrate its RNA-guided ribonuclease function. C2c2 from the bacterium Leptotrichia shahii provides interference against RNA phage. In vitro biochemical analysis shows that C2c2 is guided by a single CRISPR RNA and can be prog ...[more]