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Dose Dependent Dual Effect of Baicalin and Herb Huang Qin Extract on Angiogenesis.

ABSTRACT: Huang Qin (root of Scutellaria baicalensis) is a widely used herb in different countries for adjuvant therapy of inflammation, diabetes, hypertension, different kinds of cancer and virus related diseases. Baicalin is the main flavonoid in this herb and has been extensively studied for 30 years. The angiogenic effect of herb Huang Qin extract and baicalin was found 13 years ago, however, the results were controversial with pro-angiogenic effect in some studies and anti-angiogenic effect in others. In this paper, the angiogenic effect of baicalin, its aglycone form baicalein and aqueous extract of Huang Qin was studied in chick embryo chorioallantoic membrane (CAM) model. Dose dependent dual effect was found in both aqueous extract and baicalin, but not in baicalein, in which only inhibitory effect was observed. In order to reveal the cellular and molecular mechanism of how baicalin and baicalein affect angiogenesis, cell proliferation and programmed cell death assays were performed in treated CAM. In addition, quantitative PCR array including 84 angiogenesis related genes was used to detect high and low dosage of baicalin and baicalein responsive genes. Low dose baicalin increased cell proliferation in developing blood vessels through upregulation of multiple angiogenic genes expression, but high dose baicalin induced cell death, performing inhibitory effect on angiogenesis. Both high and low dose of baicalein down regulated the expression of multiple angiogenic genes, decreased cell proliferation, and leads to inhibitory effects on angiogenesis.

SUBMITTER: Zhu D

PROVIDER: S-EPMC5130244 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Dose Dependent Dual Effect of Baicalin and Herb Huang Qin Extract on Angiogenesis.

Zhu Dongqing D Wang Shanshan S Lawless John J He Jianchen J Zheng Zhengui Z

PloS one 20161130 11

Huang Qin (root of Scutellaria baicalensis) is a widely used herb in different countries for adjuvant therapy of inflammation, diabetes, hypertension, different kinds of cancer and virus related diseases. Baicalin is the main flavonoid in this herb and has been extensively studied for 30 years. The angiogenic effect of herb Huang Qin extract and baicalin was found 13 years ago, however, the results were controversial with pro-angiogenic effect in some studies and anti-angiogenic effect in others ...[more]

PMID: 27902752

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Project description:The concomitant use of olive leaves (OL) and glyburide (GLB) is a possible therapy for diabetic patients. However, there is no report about the effect of OL on the antidiabetic effect of GLB till now. In the current study, the possible interaction of olive leaves extract (OLE) with GLB was assessed to determine if there was any pharmacological benefit over GLB alone. Seven groups of male Sprague Dawley rats were used. Normal rats of the 1st group treated with 2?mL/kg of 3% Tween 80 (vehicle). The 2nd-5th groups were diabetic rats received vehicle, GLB (5?mg/kg), OLE low dose and OLE high dose respectively, while the 6th-7th groups administered combinations of GLB plus OLE low dose and GLB plus OLE high dose, respectively. All treatments were administered orally once daily for 8?weeks. The use of GLB+OLE-500 obviously improved fasting blood glucose (FBG), insulin and glycated hemoglobin (HbA1c) in diabetic rats (95.5?±?5.55?mg/dL, 6.8?±?0.16?mg/dL and 6.1?±?0.29%, respectively) compared to those treated with GLB monotherapy (140.0?±?6.36?mg/dL, 5.4?±?0.19?mg/dL and 7.0?±?0.20%, respectively). The lipid profile [triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C)] was significantly improved in diabetic rats exposed to GLB+OLE-500 (35.6?±?1.51?mg/dL, 48.5?±?2.74?mg/dL, 25.1?±?1.21?mg/dL and 17.0?±?0.82?mg/dL, respectively) in comparison with diabetic group exposed to GLB alone (43.2?±?2.15?mg/dL, 56.8?±?2.14?mg/dL, 18.6?±?0.96?mg/dL, 23.0?±?1.26?mg/dL, respectively). Additionally, the benefit impacts of GLB+OLE-500GLB+OLE-500 therapy on the antioxidant and lipid peroxidation parameters in the pancreatic tissues of diabetic rats were higher than those of GLB monotherapy. Moreover, GLB plus OLE-500 combination had the greatest effect on restoration of the insulin content of Beta (?) cells and reduction of the glucagon and somatostatin of Alpha (?) and Delta (?) endocrine cells in the pancreatic islets among the different treatment. The current study suggests that OL and GLB combination could cause herb-drug interactions through modulation of insulin receptor (INR), glucose transporter 2 (Slc2a2) and peroxisome proliferator-activated receptor ? (PPAR-?) genes expression in the liver of diabetic rats.

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Dose Dependent Dual Effect of Baicalin and Herb Huang Qin Extract on Angiogenesis.

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Dose Dependent Dual Effect of Baicalin and Herb Huang Qin Extract on Angiogenesis.

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