Dataset Information

Reliability of Potential Pain Biomarkers in the Saliva of Healthy Subjects: Inter-Individual Differences and Intersession Variability.

ABSTRACT:

Aim

Salivary cortisol, ?-amylase (sAA), secretory IgA (sIgA), testosterone, and soluble fraction of receptor II of TNF? (sTNF?RII) could serve as objective pain measures, but the normal variability of these potential biomarkers is unknown.

Patients & methods

Saliva was collected with the passive secretion method from 34, pain-free subjects in two single samples at least 24 hours apart. Biomarker variation and intersession reliability were assessed with the intraclass correlation coefficient (ICC). Also, we calculated the within-subject standard deviation (Sw) and the reproducibility (2.77 × Sw) of intersession measures.

Results

Salivary cortisol, sAA, sIgA, testosterone, and sTNF?RII yielded the following ICCs: 0.53, 0.003, 0.88, 0.42 and 0.83, respectively. We found no statistically significant systematic differences between sessions in any biomarker except for testosterone, which showed a decrease on the second day (p<0.001). The reproducibility for salivary cortisol, sAA, sIgA, testosterone, and sTNF?RII were 0.46 ng/ml, 12.88 U/ml, 11.7 ?g/ml, 14.54 pg/ml and 18.29 pg/ml, respectively. Cortisol, testosterone and TNF?RII measurement variability showed a positive correlation with the magnitude (p<0.002), but no relationship was found for sAA and sIgA.

Conclusions

Salivary sIgA and sTNF?RII show a remarkable good reproducibility and, therefore, could be useful as pain biomarkers. When using the passive secretion method, intersession variations in salivary sIgA of more than 11.7 ?g/ml may reflect true biomarker change. In the case of sTNF?RII this will depend of the magnitude. The estimates herein provided should help investigators and clinicians differentiate actual biomarker modification from measurement variability.

SUBMITTER: Sobas EM

PROVIDER: S-EPMC5132231 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Publications

Reliability of Potential Pain Biomarkers in the Saliva of Healthy Subjects: Inter-Individual Differences and Intersession Variability.

Sobas Eva M EM Reinoso Roberto R Cuadrado-Asensio Rubén R Fernández Itziar I Maldonado Miguel J MJ Pastor José C JC

PloS one 20161201 12

<h4>Aim</h4>Salivary cortisol, α-amylase (sAA), secretory IgA (sIgA), testosterone, and soluble fraction of receptor II of TNFα (sTNFαRII) could serve as objective pain measures, but the normal variability of these potential biomarkers is unknown.<h4>Patients & methods</h4>Saliva was collected with the passive secretion method from 34, pain-free subjects in two single samples at least 24 hours apart. Biomarker variation and intersession reliability were assessed with the intraclass correlation c ...[more]

PMID: 27907037

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Dataset Information

Reliability of Potential Pain Biomarkers in the Saliva of Healthy Subjects: Inter-Individual Differences and Intersession Variability.

Aim

Patients & methods

Results

Conclusions

Publications

Reliability of Potential Pain Biomarkers in the Saliva of Healthy Subjects: Inter-Individual Differences and Intersession Variability.

Similar Datasets

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