Ontology highlight
ABSTRACT:
SUBMITTER: Mehta AP
PROVIDER: S-EPMC5134732 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20161020 43
Several modified bases have been observed in the genomic DNA of bacteriophages, prokaryotes, and eukaryotes that play a role in restriction systems and/or epigenetic regulation. In our efforts to understand the consequences of replacing a large fraction of a canonical nucleoside with a modified nucleoside, we previously replaced around 75% of thymidine (T) with 5'-hydroxymethyl-2'-deoxyuridine (5hmU) in the Escherichia coli genome. In this study, we engineered the pyrimidine nucleotide biosynthe ...[more]