Ontology highlight
ABSTRACT:
SUBMITTER: Yang A
PROVIDER: S-EPMC5135561 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Yang Aerin A Ha Sura S Ahn Jihye J Kim Rira R Kim Sungyoon S Lee Younghoon Y Kim Jaehoon J Söll Dieter D Lee Hee-Yoon HY Park Hee-Sung HS
Science (New York, N.Y.) 20160929 6312
Many essential biological processes are controlled by posttranslational protein modifications. The inability to synthetically attain the diversity enabled by these modifications limits functional studies of many proteins. We designed a three-step approach for installing authentic posttranslational modifications in recombinant proteins. We first use the established O-phosphoserine (Sep) orthogonal translation system to create a Sep-containing recombinant protein. The Sep residue is then dephospho ...[more]