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Structure and mechanism of assembly line polyketide synthases.

ABSTRACT: Assembly line polyketide synthases (PKSs) are remarkable biosynthetic machines with considerable potential for structure-based engineering. Several types of protein-protein interactions, both within and between PKS modules, play important roles in the catalytic cycle of a multimodular PKS. Additionally, vectorial biosynthesis is enabled by the energetic coupling of polyketide chain elongation to the channeling of intermediates between successive modules. A combination of high-resolution analysis of smaller PKS components and lower resolution characterization of intact modules and bimodules has yielded insights into the structure and organization of a prototypical assembly line PKS. This review discusses our understanding of key structure-function relationships in this family of megasynthases, along with a recap of key unanswered questions in the field.

SUBMITTER: Robbins T

PROVIDER: S-EPMC5136517 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Structure and mechanism of assembly line polyketide synthases.

Robbins Thomas T Liu Yu-Chen YC Cane David E DE Khosla Chaitan C

Current opinion in structural biology 20160605

Assembly line polyketide synthases (PKSs) are remarkable biosynthetic machines with considerable potential for structure-based engineering. Several types of protein-protein interactions, both within and between PKS modules, play important roles in the catalytic cycle of a multimodular PKS. Additionally, vectorial biosynthesis is enabled by the energetic coupling of polyketide chain elongation to the channeling of intermediates between successive modules. A combination of high-resolution analysis ...[more]

PMID: 27266330

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