Dataset Information

A systematic databasing of diatoms from different geographical localities and sites of Haryana for advancing validation of forensic diatomology.

ABSTRACT: Verdict on tracing exact place of drowning is a part of medico-legal investigation. This question often stands when circumstances remain unclear about true drowning place. Usually, when a dead body rises from the bottom of drowning site, it will appear near to the point where it had actually disappeared but rapid current may carry a body to real distance from the exact place of death before any major obstruction. Forensic methodology has suggested qualitative as well as quantitative comparison of diatoms recovered in dead body and reference water samples to corroborate drowning as cause of death and locating precise place of drowning. Collection of wrong reference water samples from drowning site can also hamper the investigation process. Since, the distributions of different genera in certain extents relate particular water where the death due to drowning might have taken place; therefore, the present attempt was made to understand diatom distribution in five water bodies of Haryana with reference to diatom growth factors. This research data represents diatomological profiles of selected sites for possible application of forensic diatomology. Both, the light and scanning electron microscopy identified diatoms. It is envisioned that this data report is informative enough for the experts to plan future strategy for investigating mysteries associating place of drowning.

SUBMITTER: Saini E

PROVIDER: S-EPMC5137176 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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A systematic databasing of diatoms from different geographical localities and sites of Haryana for advancing validation of forensic diatomology.

Saini Ekta E Khanagwal V P VP Singh Rajvinder R

Data in brief 20161124

Verdict on tracing exact place of drowning is a part of medico-legal investigation. This question often stands when circumstances remain unclear about true drowning place. Usually, when a dead body rises from the bottom of drowning site, it will appear near to the point where it had actually disappeared but rapid current may carry a body to real distance from the exact place of death before any major obstruction. Forensic methodology has suggested qualitative as well as quantitative comparison o ...[more]

PMID: 27942570

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Project description:BackgroundShort tandem repeat (STR) markers play a significant role in genetic applications and have proved to be effective for the personal identification in forensic medicine. In this study, a six-dye multiplex composed of 23 autosomal STR loci (TH01, D3S1358, Penta D, D6S1043, D21S11, TPOX, D1S1656, D12S391, Penta E, D10S1248, D22S1045, D19S433, D8S1179, D2S1338, D2S441, D18S51, vWA, FGA, D16S539, CSF1PO, D13S317, D5S818, D7S820), one Y chromosome STR (DYS391), two internal quality control markers (Quality Sensor QS1 and QS2), and Amelogenin was evaluated.MethodsEvaluation studies, including PCR-based studies, sensitivity studies, species specificity studies, stability studies, DNA mixture studies, concordance studies, and precision evaluations were performed according to the guidelines of "Validation Guidelines for Forensic DNA Analysis Methods (2016)" by the Scientific Working Group on DNA Analysis Methods (SWGDAM). In addition, the forensic characteristics of 357 unrelated male samples from Han and Hui populations in China were investigated using 27 markers.ResultsFull STR profiles were obtained from different reaction volumes (5 ~ 25 μl), cycle numbers (28 ~ 34 cycles) and annealing temperatures (58 ~ 62°C). All STR profiles were obtained at humic acid concentration of up to 200 ng/μl and hematin concentration of up to 500 μM. No peaks were observed in most common animal samples except two innovative internal PCR controls (Quality Sensor QS1 and QS2). The six-dye multiplex showed a notably high value for the combined probability of exclusion (CPE), exhibiting values of with 0.99999999977688 in the Han population and 0.999999999583875 in the Hui population. The values of combined probability of discrimination (CPD) were 0.999999999999999999999999999997453 in the Han population and 0. 999999999999999999999999999994398 in the Hui population. In addition, concordance studies showed that there was no difference with the AGCU Express Marker 22 Kit.ConclusionThe results indicated that the Investigator® 26plex QS Kit is a robust, reliable, and suitable tool for forensic analysis and databasing.

Dataset Information

A systematic databasing of diatoms from different geographical localities and sites of Haryana for advancing validation of forensic diatomology.

Publications

A systematic databasing of diatoms from different geographical localities and sites of Haryana for advancing validation of forensic diatomology.

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