Ontology highlight
ABSTRACT:
SUBMITTER: Kirby N
PROVIDER: S-EPMC5137223 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Kirby Nigel N Cowieson Nathan N Hawley Adrian M AM Mudie Stephen T ST McGillivray Duncan J DJ Kusel Michael M Samardzic-Boban Vesna V Ryan Timothy M TM
Acta crystallographica. Section D, Structural biology 20161129 Pt 12
Radiation damage is a major limitation to synchrotron small-angle X-ray scattering analysis of biomacromolecules. Flowing the sample during exposure helps to reduce the problem, but its effectiveness in the laminar-flow regime is limited by slow flow velocity at the walls of sample cells. To overcome this limitation, the coflow method was developed, where the sample flows through the centre of its cell surrounded by a flow of matched buffer. The method permits an order-of-magnitude increase of X ...[more]