Ontology highlight
ABSTRACT:
SUBMITTER: Biddle W
PROVIDER: S-EPMC5137457 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Biddle Wil W Schmitt Margaret A MA Fisk John D JD
Nucleic acids research 20161023 21
Breaking the degeneracy of the genetic code via sense codon reassignment has emerged as a way to incorporate multiple copies of multiple non-canonical amino acids into a protein of interest. Here, we report the modification of a normally orthogonal tRNA by a host enzyme and show that this adventitious modification has a direct impact on the activity of the orthogonal tRNA in translation. We observed nearly equal decoding of both histidine codons, CAU and CAC, by an engineered orthogonal M. janna ...[more]