Dataset Information
Supramolecular polymer formation by cyclic dinucleotides and intercalators affects dinucleotide enzymatic processing.
Ontology highlight
ABSTRACT: Background
Cyclic dinucleotides form supramolecular aggregates with intercalators, and this property could be utilized in nanotechnology and medicine.Methods & results
Atomic force microscopy and electrophoretic mobility shift assays were used to show that cyclic diguanylic acid (c-di-GMP) forms G-wires in the presence of intercalators. The average fluorescence lifetime of thiazole orange, when bound to c-di-GMP was greater than when bound to DNA G-quadruplexes or dsDNA. The stability of c-di-GMP supramolecular polymers is dependent on both the nature of the cation present and the intercalator. C-di-GMP or cyclic diadenylic acid/intercalator complexes are more resistant to cleavage by YybT, a phosphodiesterase, than the uncomplexed nucleotides.Conclusion
Cleavage of bacterial cyclic dinucleotides could be slowed down via complexation with small molecules and that this could be utilized for diverse applications in nanotechnology and medicine.
SUBMITTER: Nakayama S
PROVIDER: S-EPMC5137846 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Publications
Supramolecular polymer formation by cyclic dinucleotides and intercalators affects dinucleotide enzymatic processing.
Future science OA 20160129 1
<h4>Background</h4>Cyclic dinucleotides form supramolecular aggregates with intercalators, and this property could be utilized in nanotechnology and medicine.<h4>Methods & results</h4>Atomic force microscopy and electrophoretic mobility shift assays were used to show that cyclic diguanylic acid (c-di-GMP) forms G-wires in the presence of intercalators. The average fluorescence lifetime of thiazole orange, when bound to c-di-GMP was greater than when bound to DNA G-quadruplexes or dsDNA. The stab ...[more]
