Bioinformatics Analysis and Validation of the Expressed Sequences Tag in Human Colorectal Adenocarcinoma.
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ABSTRACT:
SUBMITTER: Chen Y
PROVIDER: S-EPMC5139827 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Publications
Bioinformatics Analysis and Validation of the Expressed Sequences Tag in Human Colorectal Adenocarcinoma.
Chen Yao Y Zhang Chun Le CL Shen Yong Qiang YQ Wang Li Cheng LC
Gastroenterology research 20090320 2
<h4>Background</h4>This study was to investigate some new pathological genes in colorectal adenocarcinoma of human.<h4>Methods</h4>Human colorectal adenocarcinoma tissues and normal colorectal tissues were taken and suppression subtractive hybridization (SSH) and cDNA microarray techniques were employed. From differentially expressed 86 expressed sequence tags (EST), 10 EST of the SSH were selected as seed sequence for bioinformatics analyses, semi-quantitative RT-PCR and PCR-sequencing. Each la ...[more]
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