Labeling Studies Clarify the Committed Step in Bacterial Gibberellin Biosynthesis.
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ABSTRACT: Bacteria have evolved gibberellin phytohormone biosynthesis independently of plants and fungi. Through 13C-labeling and NMR analysis, the mechanistically unusual "B" ring contraction catalyzed by a cytochrome P450 (CYP114), which is the committed step in gibberellin biosynthesis, was shown to occur via oxidative extrusion of carbon-7 from ent-kaurenoic acid in bacteria. This is identical to the convergently evolved chemical transformation in plants and fungi, suggesting a common semipinacol rearrangement mechanism potentially guided by carbon-4α carboxylate proximity.
SUBMITTER: Nett RS
PROVIDER: S-EPMC5139915 | biostudies-literature |
REPOSITORIES: biostudies-literature
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