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Neucode Labels for Multiplexed, Absolute Protein Quantification.

ABSTRACT: We describe a new method to accomplish multiplexed, absolute protein quantification in a targeted fashion. The approach draws upon the recently developed neutron encoding (NeuCode) metabolic labeling strategy and parallel reaction monitoring (PRM). Since PRM scanning relies upon high-resolution tandem mass spectra for targeted protein quantification, incorporation of multiple NeuCode labeled peptides permits high levels of multiplexing that can be accessed from high-resolution tandem mass spectra. Here we demonstrate this approach in cultured cells by monitoring a viral infection and the corresponding viral protein production over many infection time points in a single experiment. In this context the NeuCode PRM combination affords up to 30 channels of quantitative information in a single MS experiment.

SUBMITTER: Potts GK

PROVIDER: S-EPMC5141612 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Neucode Labels for Multiplexed, Absolute Protein Quantification.

Potts Gregory K GK Voigt Emily A EA Bailey Derek J DJ Rose Christopher M CM Westphall Michael S MS Hebert Alexander S AS Yin John J Coon Joshua J JJ

Analytical chemistry 20160225 6

We describe a new method to accomplish multiplexed, absolute protein quantification in a targeted fashion. The approach draws upon the recently developed neutron encoding (NeuCode) metabolic labeling strategy and parallel reaction monitoring (PRM). Since PRM scanning relies upon high-resolution tandem mass spectra for targeted protein quantification, incorporation of multiple NeuCode labeled peptides permits high levels of multiplexing that can be accessed from high-resolution tandem mass spectr ...[more]

PMID: 26882330

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