Ontology highlight
ABSTRACT:
SUBMITTER: Stockman VB
PROVIDER: S-EPMC5147906 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Stockman Victoria B VB Ghamsari Lila L Lasso Gorka G Honig Barry B Shapira Sagi D SD Wang Harris H HH
PloS one 20161209 12
Comprehensive delineation of complex cellular networks requires high-throughput interrogation of genetic interactions. To address this challenge, we describe the development of a multiplex combinatorial strategy to assess pairwise genetic interactions using CRISPR-Cas9 genome editing and next-generation sequencing. We characterize the performance of combinatorial genome editing and analysis using different promoter and gRNA designs and identified regions of the chimeric RNA that are compatible w ...[more]