Ontology highlight
ABSTRACT:
SUBMITTER: Chang L
PROVIDER: S-EPMC5153662 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Chang Lingqian L Gallego-Perez Daniel D Chiang Chi-Ling CL Bertani Paul P Kuang Tairong T Sheng Yan Y Chen Feng F Chen Zhou Z Shi Junfeng J Yang Hao H Huang Xiaomeng X Malkoc Veysi V Lu Wu W Lee Ly James LJ
Small (Weinheim an der Bergstrasse, Germany) 20160920 43
While electroporation has been widely used as a physical method for gene transfection in vitro and in vivo, its application in gene therapy of cardiovascular cells remains challenging. Due to the high concentration of ion-transport proteins in the sarcolemma, conventional electroporation of primary cardiomyocytes tends to cause ion-channel activation and abnormal ion flux, resulting in low transfection efficiency and high mortality. In this work, a high-throughput nanoelectroporation technique b ...[more]