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Phylogeography of the Wheat Stem Sawfly, Cephus cinctus Norton (Hymenoptera: Cephidae): Implications for Pest Management.

ABSTRACT: The wheat stem sawfly, Cephus cinctus Norton (Hymenoptera: Cephidae), is a key pest of wheat in the northern Great Plains of North America, and damage resulting from this species has recently expanded southward. Current pest management practices are inadequate and uncertainty regarding geographic origin, as well as limited data on population structure and dynamics across North America impede progress towards more informed management. We examined the genetic divergence between samples collected in North America and northeastern Asia, the assumed native range of C. cinctus using two mitochondrial regions (COI and 16S). Subsequently, we characterized the structure of genetic diversity in the main wheat producing areas in North America using a combination of mtDNA marker and microsatellites in samples collected both in wheat fields and in grasses in wildlands. The strong genetic divergence observed between North American samples and Asian congeners, in particular the synonimized C. hyalinatus, did not support the hypothesis of a recent American colonization by C. cinctus. Furthermore, the relatively high genetic diversity both with mtDNA and microsatellite markers offered additional evidence in favor of the native American origin of this pest. The genetic diversity of North American populations is structured into three genetic clusters and these are highly correlated with geography. Regarding the recent southern outbreaks in North America, the results tend to exclude the hypothesis of recent movement of damaging wheat stem sawfly populations from the northern area. The shift in host plant use by local populations appears to be the most likely scenario. Finally, the significance of these findings is discussed in the context of pest management.

SUBMITTER: Lesieur V

PROVIDER: S-EPMC5154603 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Accurately estimating cause-specific mortality for immature insect herbivores is usually difficult. The insects are exposed to abiotic and biotic mortality factors, causing cadavers to simply disappear before cause of mortality can be recorded. Also, insect herbivores are often highly mobile on hosts, making it difficult to follow patterns for individuals through time. In contrast, the wheat stem sawfly, Cephus cinctus Norton, spends its entire egg, larval, and pupal period inside a host stem. Therefore, with periodic sampling stage-specific causes of mortality can be ascertained. Consequently, we examined C. cinctus mortality in eight barley, Hordeum vulgare L., cultivars in two locations in Montana from 2016 to 2018 by collecting stem samples from stem elongation to crop maturity at weekly intervals, and collecting overwintered barley stubs the following spring and summer from the same plots. If larvae were present, we examined larval status-dead or alive-and categorized dead individuals into one of 5 mortality categories: plant defense, cannibalism, parasitism, pathogens, and unknown factors. We used multiple decrement life tables to estimate cause-specific mortality and irreplaceable mortality (the proportion of mortality from a given cause that cannot be replaced by other causes of mortality). Plant defense (antibiosis) caused 85.7 ± 3.6%, cannibalism (governed by antixenosis) caused 70.1 ± 7.6%, parasitism caused 13.8 ± 5.9%, unknown factors caused 38.5 ± 7.6%, and pathogens caused 14.7 ± 8.5% mortality in the presence of all causes of mortality. Similarly, irreplaceable mortality due to plant defense was 22.3 ± 6.4%, cannibalism was 29.1± 4.2%, unknown factors was 6.2 ± 1.8%, pathogens was 0.9 ± 0.5%, and parasitism was 1. 5 ± 0. 6%. Antibiosis traits primarily killed newly emerged larvae, while other traits supported more favorable oviposition decisions by females, increasing mortality by obligate cannibalism. Our results suggest that breeding barley for resistance to C. cinctus targeting both categories of traits (antibiosis and antixenosis) is a highly valuable tactic for management of this important pest.

Project description:IntroductionWheat stem sawfly (WSS), Cephus cinctus Norton, is a major pest of common bread wheat (Triticum aestivum L.) and other cultivated cereals in North America. Planting of cultivars with solid stems has been the primary management strategy to prevent yield loss due to WSS infestation, however expression of this phenotype can vary depending on environmental conditions and solid stems hinder biological control of WSS via braconid parasitoids Bracon cephi (Gahan) and Bracon lissogaster Muesebeck. In the hollow stems of oat (Avena sativa L.), WSS larvae experience 100% mortality before they reach late instars, but the mechanisms for this observed resistance have not been characterized.ObjectiveThe objective of this study was to explore additional sources of resistance outside of the historic solid stem phenotype.MethodsHere, we use an untargeted metabolomics approach to examine the response of the metabolome of two cultivars of oat and four cultivars of spring wheat to infestation by WSS. Using liquid chromatography-mass spectrometry (LC-MS), differentially expressed metabolites were identified between oat and wheat which were associated with the phenylpropanoid pathway, phospholipid biosynthesis and signaling, the salicylic acid signaling pathway, indole-3-acetic acid (IAA) degradation, and biosynthesis of 1,4-benzoxazin-3-ones (Bxs). Several phospho- and galacto- lipids were found in higher abundance in oat, and with the exception of early stem solidness cultivar Conan, both species experienced a decrease in abundance once infested. In all wheat cultivars except Conan, an increase in abundance was observed for Bxs HMDBOA-glc and DIBOA-β-D-glucoside after infestation, indicating that this pathway is involved in wheat response to infestation in both solid and hollow stemmed cultivars. Differences between species in compounds involved in IAA biosynthesis, degradation and inactivation suggest that wheat may respond to infestation by inactivating IAA or altering the IAA pool in stem tissue.ConclusionWe propose that the species differences found here likely affect the survival of WSS larvae and may also be associated with differences in stem architecture at the molecular level. Our findings suggest pathways to focus on for future studies in elucidating plant response to WSS infestation.

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Phylogeography of the Wheat Stem Sawfly, Cephus cinctus Norton (Hymenoptera: Cephidae): Implications for Pest Management.

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