Ontology highlight
ABSTRACT:
SUBMITTER: Okkelman IA
PROVIDER: S-EPMC5156356 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
PloS one 20161214 12
Incorporation of thymidine analogues in replicating DNA, coupled with antibody and fluorophore staining, allows analysis of cell proliferation, but is currently limited to monolayer cultures, fixed cells and end-point assays. We describe a simple microscopy imaging method for live real-time analysis of cell proliferation, S phase progression over several division cycles, effects of anti-proliferative drugs and other applications. It is based on the prominent (~ 1.7-fold) quenching of fluorescenc ...[more]