Ontology highlight
ABSTRACT:
SUBMITTER: Thomes PG
PROVIDER: S-EPMC5156597 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Thomes Paul G PG Brandon-Warner Elizabeth E Li Ting T Donohue Terrence M TM Schrum Laura W LW
Data in brief 20161208
Our data describe autophagic flux in primary rat hepatic stellate cells (rHSCs) treated with pro-fibrotic growth factor, transforming growth factor beta (TGF-β). An autophagy flux experiment determines the rate of synthesis and degradation of the autophagosome marker, LC3-II in the presence and absence of the lysosomal inhibitor bafilomcyin, which blocks LC3-II degradation in lysosomes. The effects of a test agent on LC3-II flux through the autophagic pathway is determined immunochemically by it ...[more]