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Screening and purification of catechins from underutilized tea plant parts and their bioactivity studies.


ABSTRACT: Comparative investigation of major phytoconstituents was performed from various parts of tea plant viz. apical bud, subtending 1st-5th leaf, stem, coarse leaves, flowers, fruits and roots. From the results of comparative RP-HPLC-DAD analysis it was found that underutilized tea parts especially coarse leaves, flowers and fruits contains abundant amount of phenolics (17.5%) and catechins (4-5%). From these underutilized tea plant parts the catechins were extracted and purified and then screened for their anticancer, immunomodulatory effect and antimicrobial activity against food borne pathogens. The results showed that tea fruit extract exhibited higher toxicity against oral cancer cells and also promotes proliferation of mice splenocytes. The results of antimicrobial studies revealed the inhibitory effect of these extracts against both gram positive and gram negative bacteria. These investigations clearly demonstrated that the underutilized tea plant parts could act as economical and sustainable bioresource of functionally active constituents which further lead to the development of new cost-effective nutraceuticals and other formulations.

SUBMITTER: Rana A 

PROVIDER: S-EPMC5156646 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Screening and purification of catechins from underutilized tea plant parts and their bioactivity studies.

Rana Ajay A   Sharma Eshita E   Rawat Kiran K   Sharma Ranjana R   Verma Sarika S   Padwad Yogendra Y   Gulati Ashu A  

Journal of food science and technology 20161126 11


Comparative investigation of major phytoconstituents was performed from various parts of tea plant viz. apical bud, subtending 1st-5th leaf, stem, coarse leaves, flowers, fruits and roots. From the results of comparative RP-HPLC-DAD analysis it was found that underutilized tea parts especially coarse leaves, flowers and fruits contains abundant amount of phenolics (17.5%) and catechins (4-5%). From these underutilized tea plant parts the catechins were extracted and purified and then screened fo  ...[more]

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