Dataset Information

Enabling the Triplet of Tetraphenylethene to Sensitize the Excited State of Europium(III) for Protein Detection and Time-Resolved Luminescence Imaging.

ABSTRACT: A tetraphenylethene (TPE) group that exhibits aggregation-induced emission is incorporated into the ligand of a Eu(III) complex (TPEEu) to sensitize the excited state of Eu(III). In steady-state measurements, TPEEu exhibits weak luminescence when dissolved in aqueous solutions even at a high concentration level, but emits strong fluorescence of TPE and phosphorescence of Eu(III) upon binding with bovine serum albumin. With a delay time of 0.05 ms and a gate time of 1.0 ms in time-resolved measurements, only phosphorescent emission of Eu(III) is observed with a high on/off ratio. Moreover, this probe is successfully used in time-resolved luminescence imaging to eliminate the background signal from biological autofluorescence without a washing process. This work provides a general strategy in designing Ln(III) complexes for detecting a broad range of biological molecules.

SUBMITTER: Zhu Z

PROVIDER: S-EPMC5157173 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Enabling the Triplet of Tetraphenylethene to Sensitize the Excited State of Europium(III) for Protein Detection and Time-Resolved Luminescence Imaging.

Zhu Zece Z Song Bo B Yuan Jingli J Yang Chuluo C

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20160712 12

A tetraphenylethene (TPE) group that exhibits aggregation-induced emission is incorporated into the ligand of a Eu(III) complex (TPEEu) to sensitize the excited state of Eu(III). In steady-state measurements, TPEEu exhibits weak luminescence when dissolved in aqueous solutions even at a high concentration level, but emits strong fluorescence of TPE and phosphorescence of Eu(III) upon binding with bovine serum albumin. With a delay time of 0.05 ms and a gate time of 1.0 ms in time-resolved measur ...[more]

PMID: 27981006

Similar Datasets

Project description:The structure of 30 monosubstituted benzenes in the first excited triplet T1 state was optimized with both unrestricted (U) and restricted open shell (RO) approximations combined with the ?B97XD/aug-cc-pVTZ basis method. The substituents exhibited diverse ?- and ?-electron-donating and/or -withdrawing groups. Two different positions of the substituents are observed in the studied compounds in the T1 state: one distorted from the plane and the other coplanar with a quinoidal ring. The majority of the substituents are ?-electron donating in the first group while ?-electron withdrawing in the second one. Basically, U- and RO-?B97XD approximations yield concordant results except for the B-substituents and a few of the planar groups. In the T1 state, the studied molecules are not aromatic, yet aromaticity estimated using the HOMA (harmonic oscillator model of aromaticity) index increases from ca. -0.2 to ca. 0.4 with substituent distortion, while in the S1 state, they are only slightly less aromatic than in the ground state (HOMA ?0.8 vs ?1.0, respectively). Unexpectedly, the sEDA(T1) and pEDA(T1) substituent effect descriptors do not correlate with analogous parameters for the ground and first excited singlet states. This is because in the T1 state, the geometry of the ring changes dramatically and the sEDA(T1) and pEDA(T1) descriptors do not characterize only the functional group but the entire molecule. Thus, they cannot provide useful scales for the substituents in the T1 states. We found that the spin density in the T1 states is accumulated at the Cipso and Cp atoms, and with the substituent deformation angle, it nonlinearly increases at the former while decreases at the latter. It appeared that the gap between singly unoccupied molecular orbital and singly occupied molecular orbital (SUMO-SOMO) is determined by the change of the SOMO energy because the former is essentially constant. For the nonplanar structures, SOMO correlates with the torsion angle of the substituent and the ground-state pEDA(S0) descriptor of the ?-electron-donating substituents ranging from 0.02 to 0.2 e. Finally, shapes of the SOMO-1 instead of SOMO frontier orbitals in the T1 state somehow resemble the highest occupied molecular orbital ones of the S0 and S1 states. For several planar systems, the shape of the U- and RO-density functional theory-calculated SOMO-1 orbitals differs substantially.

Dataset Information

Enabling the Triplet of Tetraphenylethene to Sensitize the Excited State of Europium(III) for Protein Detection and Time-Resolved Luminescence Imaging.

Publications

Enabling the Triplet of Tetraphenylethene to Sensitize the Excited State of Europium(III) for Protein Detection and Time-Resolved Luminescence Imaging.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets