Ontology highlight
ABSTRACT:
SUBMITTER: Hanhijarvi KJ
PROVIDER: S-EPMC5159644 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Hanhijärvi Kalle J KJ Ziedaite Gabija G Bamford Dennis H DH Hæggström Edward E Poranen Minna M MM
RNA (New York, N.Y.) 20161101 1
Genome packaging of double-stranded RNA (dsRNA) phages has been widely studied using biochemical and molecular biology methods. We adapted the existing in vitro packaging system of one such phage for single-molecule experimentation. To our knowledge, this is the first attempt to study the details of viral RNA packaging using optical tweezers. Pseudomonas phage φ6 is a dsRNA virus with a tripartite genome. Positive-sense (+) single-stranded RNA (ssRNA) genome precursors are packaged into a prefor ...[more]