Ontology highlight
ABSTRACT:
SUBMITTER: Gallego-Perez D
PROVIDER: S-EPMC5161095 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Gallego-Perez Daniel D Otero Jose J JJ Czeisler Catherine C Ma Junyu J Ortiz Cristina C Gygli Patrick P Catacutan Fay Patsy FP Gokozan Hamza Numan HN Cowgill Aaron A Sherwood Thomas T Ghatak Subhadip S Malkoc Veysi V Zhao Xi X Liao Wei-Ching WC Gnyawali Surya S Gnyawali Surya S Wang Xinmei X Adler Andrew F AF Leong Kam K Wulff Brian B Wilgus Traci A TA Askwith Candice C Khanna Savita S Rink Cameron C Sen Chandan K CK Lee L James LJ
Nanomedicine : nanotechnology, biology, and medicine 20151219 2
Safety concerns and/or the stochastic nature of current transduction approaches have hampered nuclear reprogramming's clinical translation. We report a novel non-viral nanotechnology-based platform permitting deterministic large-scale transfection with single-cell resolution. The superior capabilities of our technology are demonstrated by modification of the well-established direct neuronal reprogramming paradigm using overexpression of the transcription factors Brn2, Ascl1, and Myt1l (BAM). Rep ...[more]