Dataset Information

Bronchoarterial ratio in never-smokers adults: Implications for bronchial dilation definition.

ABSTRACT: Bronchiectasis manifests as recurrent respiratory infections and reduced lung function. Airway dilation, which is measured as the ratio of the diameters of the bronchial lumen (B) and adjacent pulmonary artery (A), is a defining radiological feature of bronchiectasis. A challenge to equating the bronchoarterial (BA) ratio to disease severity is that the diameters of airway and vessel in health are not established. We sought to explore the variability of BA ratio in never-smokers without pulmonary disease and its associations with lung function.Objective measurements of the BA ratio on volumetric computed tomography (CT) scans and pulmonary function data were collected in 106 never-smokers. The BA ratio was measured in the right upper lobe apical bronchus (RB1) and the right lower lobe basal posterior bronchus. The association between the BA ratio and forced expiratory volume in 1?s (FEV1 ) was assessed using regression analysis.The BA ratio was 0.79?±?0.16 and was smaller in more peripheral RB1 bronchi (P?1, a typical threshold for bronchiectasis, in 10 (8.5%) subjects. Subjects with a BA ratio >1 versus ?1 had smaller artery diameters (P?1 is driven by small arteries. Using artery diameter as reference to define bronchial dilation seems inappropriate.

SUBMITTER: Diaz AA

PROVIDER: S-EPMC5161710 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Bronchoarterial ratio in never-smokers adults: Implications for bronchial dilation definition.

Diaz Alejandro A AA Young Thomas P TP Maselli Diego J DJ Martinez Carlos H CH Maclean Erick S ES Yen Andrew A Dass Chandra C Simpson Scott A SA Lynch David A DA Kinney Gregory L GL Hokanson John E JE Washko George R GR San José Estépar Raul R

Respirology (Carlton, Vic.) 20160818 1

<h4>Background and objective</h4>Bronchiectasis manifests as recurrent respiratory infections and reduced lung function. Airway dilation, which is measured as the ratio of the diameters of the bronchial lumen (B) and adjacent pulmonary artery (A), is a defining radiological feature of bronchiectasis. A challenge to equating the bronchoarterial (BA) ratio to disease severity is that the diameters of airway and vessel in health are not established. We sought to explore the variability of BA ratio ...[more]

PMID: 27538197

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Project description:BackgroundAlthough prior studies have demonstrated a smoking-induced field of molecular injury throughout the lung and airway, the impact of smoking on the airway epithelial proteome and its relationship to smoking-related changes in the airway transcriptome are unclear.Methodology/principal findingsAirway epithelial cells were obtained from never (n = 5) and current (n = 5) smokers by brushing the mainstem bronchus. Proteins were separated by one dimensional polyacrylamide gel electrophoresis (1D-PAGE). After in-gel digestion, tryptic peptides were processed via liquid chromatography/ tandem mass spectrometry (LC-MS/MS) and proteins identified. RNA from the same samples was hybridized to HG-U133A microarrays. Protein detection was compared to RNA expression in the current study and a previously published airway dataset. The functional properties of many of the 197 proteins detected in a majority of never smokers were similar to those observed in the never smoker airway transcriptome. LC-MS/MS identified 23 proteins that differed between never and current smokers. Western blotting confirmed the smoking-related changes of PLUNC, P4HB1, and uteroglobin protein levels. Many of the proteins differentially detected between never and current smokers were also altered at the level of gene expression in this cohort and the prior airway transcriptome study. There was a strong association between protein detection and expression of its corresponding transcript within the same sample, with 86% of the proteins detected by LC-MS/MS having a detectable corresponding probeset by microarray in the same sample. Forty-one proteins identified by LC-MS/MS lacked detectable expression of a corresponding transcript and were detected in <or=5% of airway samples from a previously published dataset.Conclusions/significance1D-PAGE coupled with LC-MS/MS effectively profiled the airway epithelium proteome and identified proteins expressed at different levels as a result of cigarette smoke exposure. While there was a strong correlation between protein and transcript detection within the same sample, we also identified proteins whose corresponding transcripts were not detected by microarray. This noninvasive approach to proteomic profiling of airway epithelium may provide additional insights into the field of injury induced by tobacco exposure.

Dataset Information

Bronchoarterial ratio in never-smokers adults: Implications for bronchial dilation definition.

Publications

Bronchoarterial ratio in never-smokers adults: Implications for bronchial dilation definition.

Similar Datasets

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