Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA.
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ABSTRACT:
SUBMITTER: Weldon C
PROVIDER: S-EPMC5164935 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Publications
Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA.
Weldon Carika C Behm-Ansmant Isabelle I Hurley Laurence H LH Burley Glenn A GA Branlant Christiane C Eperon Ian C IC Dominguez Cyril C
Nature chemical biology 20161107 1
RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions. ...[more]
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