Ontology highlight
ABSTRACT:
SUBMITTER: Voss S
PROVIDER: S-EPMC5167155 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Voss Stephanie S Krüger Dennis M DM Koch Oliver O Wu Yao-Wen YW
Proceedings of the National Academy of Sciences of the United States of America 20161129 50
Ras-like small GTPases function as molecular switches and regulate diverse cellular events. To examine the dynamics of signaling requires spatiotemporal visualization of their activity in the cell. Current small GTPase sensors rely on specific effector domains that are available for only a small number of GTPases and compete for endogenous regulator/effector binding. Here, we describe versatile conformational sensors for GTPase activity (COSGAs) based on the conserved GTPase fold. Conformational ...[more]