Ontology highlight
ABSTRACT:
SUBMITTER: Mogollon CM
PROVIDER: S-EPMC5172577 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Mogollon Catherin Marin CM van Pul Fiona J A FJ Imai Takashi T Ramesar Jai J Chevalley-Maurel Séverine S de Roo Guido M GM Veld Sabrina A J SA Kroeze Hans H Franke-Fayard Blandine M D BM Janse Chris J CJ Khan Shahid M SM
PloS one 20161220 12
The CRISPR/Cas9 system is a powerful genome editing technique employed in a wide variety of organisms including recently the human malaria parasite, P. falciparum. Here we report on further improvements to the CRISPR/Cas9 transfection constructs and selection protocol to more rapidly modify the P. falciparum genome and to introduce transgenes into the parasite genome without the inclusion of drug-selectable marker genes. This method was used to stably integrate the gene encoding GFP into the P. ...[more]