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Inter-sigmulon communication through topological promoter coupling.

ABSTRACT: Divergent transcription from within bacterial intergenic regions frequently involves promoters dependent on alternative ?-factors. This is the case for the non-overlapping ?⁷⁰- and ?⁵⁴-dependent promoters that control production of the substrate-responsive regulator and enzymes for (methyl)phenol catabolism. Here, using an array of in vivo and in vitro assays, we identify transcription-driven supercoiling arising from the ?⁵⁴-promoter as the mechanism underlying inter-promoter communication that results in stimulation of the activity of the ?⁷⁰-promoter. The non-overlapping 'back-to-back' configuration of a powerful ?⁵⁴-promoter and weak ?⁷⁰-promoter within this system offers a previously unknown means of inter-sigmulon communication that renders the ?⁷⁰-promoter subservient to signals that elicit ?⁵⁴-dependent transcription without it possessing a cognate binding site for the ?⁵⁴-RNA polymerase holoenzyme. This mode of control has the potential to be a prevalent, but hitherto unappreciated, mechanism by which bacteria adjust promoter activity to gain appropriate transcriptional control.

SUBMITTER: Del Peso Santos T

PROVIDER: S-EPMC5175336 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Inter-sigmulon communication through topological promoter coupling.

Del Peso Santos Teresa T Shingler Victoria V

Nucleic acids research 20160715 20

Divergent transcription from within bacterial intergenic regions frequently involves promoters dependent on alternative σ-factors. This is the case for the non-overlapping σ70- and σ54-dependent promoters that control production of the substrate-responsive regulator and enzymes for (methyl)phenol catabolism. Here, using an array of in vivo and in vitro assays, we identify transcription-driven supercoiling arising from the σ54-promoter as the mechanism underlying ...[more]

PMID: 27422872

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Project description:Inter-cellular and inter-kingdom signaling systems of various levels of complexity regulate pathogenic and mutualistic interactions between bacteria, parasites, and fungi and animal and plant hosts. Inter-kingdom interactions between mutualistic bacteria such as rhizobia and legumes during nodulation and between fungi and plants during mycorrhizal associations, are characterized by the extensive exchange of molecular signals, which allow nitrogen and phosphate assimilation, respectively. A novel aspect of this signaling exchange is the existence of specific structures, the exosomes, that carry important molecules that shape the plant-pathogen interactions. Exosomes contain a wide array of molecules, such as lipids, proteins, messenger RNA, and microRNAs, that play important roles in cell-to-cell communication in animal and plant cells by affecting gene expression and other physiological activity in distant cells within the same organism (e.g., during cancer metastases and neuron injuries). In plant cells, it has been recently reported that exosomes go beyond organism boundaries and inhibit a pathogenic interaction in plants. Plant produce and send exosomes loaded with specific small miRNA which inhibit the pathogen infection, but the pathogen can also produce exosomes carrying pro-pathogenic proteins and microRNAs. Therefore, exosomes are the important bridge regulating the signal exchange. Exosomes are small membrane-bound vesicles derived from multivesicular bodies (MVBs), which carries selected cargos from the cytoplasm (protein, lipids, and microRNAs) and under certain circumstances, they fuse with the plasma membrane, releasing the small vesicles as cargo-carrying exosomes into the extracellular space during intercellular and inter-kingdom communication. Animal and plant proteomic studies have demonstrated that tetraspanin proteins are an integral part of exosome membranes, positioning tetraspanins as essential components for endosome organization, with key roles in membrane fusion, cell trafficking, and membrane recognition. We discuss the similarities and differences between animal tetraspanins and plant tetraspanins formed during plant-microbe interactions and their potential role in mutualistic communication.

Dataset Information

Inter-sigmulon communication through topological promoter coupling.

Publications

Inter-sigmulon communication through topological promoter coupling.

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