Inter-sigmulon communication through topological promoter coupling.

ABSTRACT: Divergent transcription from within bacterial intergenic regions frequently involves promoters dependent on alternative σ-factors. This is the case for the non-overlapping σ⁷⁰- and σ⁵⁴-dependent promoters that control production of the substrate-responsive regulator and enzymes for (methyl)phenol catabolism. Here, using an array of in vivo and in vitro assays, we identify transcription-driven supercoiling arising from the σ⁵⁴-promoter as the mechanism underlying inter-promoter communication that results in stimulation of the activity of the σ⁷⁰-promoter. The non-overlapping 'back-to-back' configuration of a powerful σ⁵⁴-promoter and weak σ⁷⁰-promoter within this system offers a previously unknown means of inter-sigmulon comm

SUBMITTER: Del Peso Santos T 

PROVIDER: S-EPMC5175336 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature