Dataset Information

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.

ABSTRACT: Whole exome sequencing (WES) accelerates disease gene discovery using rare genetic variants, but further statistical and functional evidence is required to avoid false-discovery. To complement variant-driven disease gene discovery, here we present function-driven disease gene discovery in zebrafish (Danio rerio), a promising human disease model owing to its high anatomical and genomic similarity to humans. To facilitate zebrafish-based function-driven disease gene discovery, we developed a genome-scale co-functional network of zebrafish genes, DanioNet (www.inetbio.org/danionet), which was constructed by Bayesian integration of genomics big data. Rigorous statistical assessment confirmed the high prediction capacity of DanioNet for a wide variety of human diseases. To demonstrate the feasibility of the function-driven disease gene discovery using DanioNet, we predicted genes for ciliopathies and performed experimental validation for eight candidate genes. We also validated the existence of heterozygous rare variants in the candidate genes of individuals with ciliopathies yet not in controls derived from the UK10K consortium, suggesting that these variants are potentially involved in enhancing the risk of ciliopathies. These results showed that an integrated genomics big data for a model animal of diseases can expand our opportunity for harnessing WES data in disease gene discovery.

SUBMITTER: Shim H

PROVIDER: S-EPMC5175370 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.

Shim Hongseok H Kim Ji Hyun JH Kim Chan Yeong CY Hwang Sohyun S Kim Hyojin H Yang Sunmo S Lee Ji Eun JE Lee Insuk I

Nucleic acids research 20161005 20

Whole exome sequencing (WES) accelerates disease gene discovery using rare genetic variants, but further statistical and functional evidence is required to avoid false-discovery. To complement variant-driven disease gene discovery, here we present function-driven disease gene discovery in zebrafish (Danio rerio), a promising human disease model owing to its high anatomical and genomic similarity to humans. To facilitate zebrafish-based function-driven disease gene discovery, we developed a genom ...[more]

PMID: 27903883

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Dataset Information

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.

Publications

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.

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