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Identification and characterization of Daldinia eschscholtzii isolated from skin scrapings, nails, and blood.


ABSTRACT:

Background

Daldinia eschscholtzii is a filamentous wood-inhabiting endophyte commonly found in woody plants. Here, we report the identification and characterization of nine D. eschscholtzii isolates from skin scrapings, nail clippings, and blood.

Methods

The nine isolates were identified based on colony morphology, light microscopy, and internal transcribed spacer (ITS)-based phylogeny. In vitro antifungal susceptibility of the fungal isolates was evaluated by the Etest to determine the minimum inhibitory concentration (MIC).

Results

The nine isolates examined were confirmed as D. eschscholtzii. They exhibited typical features of Daldinia sp. on Sabouraud Dextrose Agar, with white felty colonies and black-gray coloration on the reverse side. Septate hyphae, branching conidiophore with conidiogenous cells budding from its terminus, and nodulisporium-like conidiophores were observed under the microscope. Phylogenetic analysis revealed that the nine isolates were clustered within the D. eschscholtzii species complex. All the isolates exhibited low MICs against azole agents (voriconazole, posaconazole, itraconazole, and ketoconazole), as well as amphotericin B, with MIC of less than 1 µg/ml.

Discussion

Early and definitive identification of D. eschscholtzii is vital to reducing misuse of antimicrobial agents. Detailed morphological and molecular characterization as well as antifungal profiling of D. eschscholtzii provide the basis for future studies on its biology, pathogenicity, and medicinal potential.

SUBMITTER: Ng KP 

PROVIDER: S-EPMC5178343 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Publications

Identification and characterization of <i>Daldinia eschscholtzii</i> isolated from skin scrapings, nails, and blood.

Ng Kee Peng KP   Chan Chai Ling CL   Yew Su Mei SM   Yeo Siok Koon SK   Toh Yue Fen YF   Looi Hong Keat HK   Na Shiang Ling SL   Lee Kok Wei KW   Yee Wai-Yan WY   Kuan Chee Sian CS  

PeerJ 20161220


<h4>Background</h4><i>Daldinia eschscholtzii</i> is a filamentous wood-inhabiting endophyte commonly found in woody plants. Here, we report the identification and characterization of nine <i>D. eschscholtzii</i> isolates from skin scrapings, nail clippings, and blood.<h4>Methods</h4>The nine isolates were identified based on colony morphology, light microscopy, and internal transcribed spacer (ITS)-based phylogeny. <i>In vitro</i> antifungal susceptibility of the fungal isolates was evaluated by  ...[more]

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