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ABSTRACT: Background
Daldinia eschscholtzii is a filamentous wood-inhabiting endophyte commonly found in woody plants. Here, we report the identification and characterization of nine D. eschscholtzii isolates from skin scrapings, nail clippings, and blood.Methods
The nine isolates were identified based on colony morphology, light microscopy, and internal transcribed spacer (ITS)-based phylogeny. In vitro antifungal susceptibility of the fungal isolates was evaluated by the Etest to determine the minimum inhibitory concentration (MIC).Results
The nine isolates examined were confirmed as D. eschscholtzii. They exhibited typical features of Daldinia sp. on Sabouraud Dextrose Agar, with white felty colonies and black-gray coloration on the reverse side. Septate hyphae, branching conidiophore with conidiogenous cells budding from its terminus, and nodulisporium-like conidiophores were observed under the microscope. Phylogenetic analysis revealed that the nine isolates were clustered within the D. eschscholtzii species complex. All the isolates exhibited low MICs against azole agents (voriconazole, posaconazole, itraconazole, and ketoconazole), as well as amphotericin B, with MIC of less than 1 µg/ml.Discussion
Early and definitive identification of D. eschscholtzii is vital to reducing misuse of antimicrobial agents. Detailed morphological and molecular characterization as well as antifungal profiling of D. eschscholtzii provide the basis for future studies on its biology, pathogenicity, and medicinal potential.
SUBMITTER: Ng KP
PROVIDER: S-EPMC5178343 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
PeerJ 20161220
<h4>Background</h4><i>Daldinia eschscholtzii</i> is a filamentous wood-inhabiting endophyte commonly found in woody plants. Here, we report the identification and characterization of nine <i>D. eschscholtzii</i> isolates from skin scrapings, nail clippings, and blood.<h4>Methods</h4>The nine isolates were identified based on colony morphology, light microscopy, and internal transcribed spacer (ITS)-based phylogeny. <i>In vitro</i> antifungal susceptibility of the fungal isolates was evaluated by ...[more]