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SLBP is associated with histone mRNA on polyribosomes as a component of the histone mRNP.


ABSTRACT: The stem-loop binding protein (SLBP) binds the 3' end of histone mRNA and is present both in nucleus, and in the cytoplasm on the polyribosomes. SLBP participates in the processing of the histone pre-mRNA and in translation of the mature message. Histone mRNAs are rapidly degraded when cells are treated with inhibitors of DNA replication and are stabilized by inhibitors of translation, resulting in an increase in histone mRNA levels. Here, we show that SLBP is a component of the histone messenger ribonucleoprotein particle (mRNP). Histone mRNA from polyribosomes is immunoprecipitated with anti-SLBP. Most of the SLBP in cycloheximide-treated cells is present on polyribosomes as a result of continued synthesis and transport of the histone mRNP to the cytoplasm. When cells are treated with inhibitors of DNA replication, histone mRNAs are rapidly degraded but SLBP levels remain constant and SLBP is relocalized to the nucleus. SLBP remains active both in RNA binding and histone pre-mRNA processing when DNA replication is inhibited.

SUBMITTER: Whitfield ML 

PROVIDER: S-EPMC519100 | biostudies-literature | 2004

REPOSITORIES: biostudies-literature

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SLBP is associated with histone mRNA on polyribosomes as a component of the histone mRNP.

Whitfield Michael L ML   Kaygun Handan H   Erkmann Judith A JA   Townley-Tilson W H Davin WH   Dominski Zbigniew Z   Marzluff William F WF  

Nucleic acids research 20040909 16


The stem-loop binding protein (SLBP) binds the 3' end of histone mRNA and is present both in nucleus, and in the cytoplasm on the polyribosomes. SLBP participates in the processing of the histone pre-mRNA and in translation of the mature message. Histone mRNAs are rapidly degraded when cells are treated with inhibitors of DNA replication and are stabilized by inhibitors of translation, resulting in an increase in histone mRNA levels. Here, we show that SLBP is a component of the histone messenge  ...[more]

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