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Mechanical Criterion for the Rupture of a Cell Membrane under Compression.

ABSTRACT: We investigate the mechanical conditions leading to the rupture of the plasma membrane of an endothelial cell subjected to a local, compressive force. Membrane rupture is induced by tilted microindentation, a technique used to perform mechanical measurements on adherent cells. In this technique, the applied force can be deduced from the measured horizontal displacement of a microindenter's tip, as imaged with an inverted microscope and without the need for optical sensors to measure the microindenter's deflection. We show that plasma membrane rupture of endothelial cells occurs at a well-defined value of the applied compressive stress. As a point of reference, we use numerical simulations to estimate the magnitude of the compressive stresses exerted on endothelial cells during the deployment of a stent.

SUBMITTER: Gonzalez-Rodriguez D

PROVIDER: S-EPMC5192693 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Mechanical Criterion for the Rupture of a Cell Membrane under Compression.

Gonzalez-Rodriguez David D Guillou Lionel L Cornat François F Lafaurie-Janvore Julie J Babataheri Avin A de Langre Emmanuel E Barakat Abdul I AI Husson Julien J

Biophysical journal 20161201 12

We investigate the mechanical conditions leading to the rupture of the plasma membrane of an endothelial cell subjected to a local, compressive force. Membrane rupture is induced by tilted microindentation, a technique used to perform mechanical measurements on adherent cells. In this technique, the applied force can be deduced from the measured horizontal displacement of a microindenter's tip, as imaged with an inverted microscope and without the need for optical sensors to measure the microind ...[more]

PMID: 28002747

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