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Expression of genes involved in brain GABAergic neurotransmission in three-spined stickleback exposed to near-future CO2.

ABSTRACT: Change in the activity of the main inhibitory receptor, GABAA, has been suggested to be a general mechanism behind the behavioural alterations reported in ocean acidification studies on fish. It has been proposed that regulatory acid-base mechanisms in response to high CO2 alter the neuronal Cl- and HCO3- gradients that are important for GABAA receptor function. Here, we report a comprehensive analysis of gene expression of GABAA receptor subunits and of genes involved in GABAergic transmission in the brain of fish exposed to near-future CO2. Altogether, 56 mRNA transcripts were quantified in brains of three-spined stickleback (Gasterosteus aculeatus) kept in control pCO2 (333 ± 30 ?atm CO2) or at high pCO2 levels (991 ± 57 ?atm) for 43 days. The gene expression analysis included GABAA receptor subunits (?1-6, ?1-3, ?1-3, ?, ? and ?1-3), enzymes and transporters involved in GABA metabolism (GAD1-2, GABAT and GAT1-3), GABAA receptor-associated proteins (GABARAP and GABARAPL), ion cotransporters (KCC1-4, NKCC1, ClC21-3, AE3 and NDAE) and carbonic anhydrase (CAII). Exposure to high CO2 had only minor effects on the expression of genes involved in GABAergic neurotransmission. There were significant increases in the mRNA levels of ? family subunits of the GABAA receptor, with a more pronounced expression of ?12, ?3, ?4 and ?6b. No changes were detected in the expression of other GABAA subunits or in genes related to receptor turnover, GABA metabolism or ion transport. Although the minor changes seen for mRNA levels might reflect compensatory mechanisms in the high-CO2 conditions, these were apparently insufficient to restore normal neural function, because the behavioural changes persisted within the time frame studied.

SUBMITTER: Lai F

PROVIDER: S-EPMC5196030 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Expression of genes involved in brain GABAergic neurotransmission in three-spined stickleback exposed to near-future CO2.

Lai Floriana F Fagernes Cathrine E CE Jutfelt Fredrik F Nilsson Göran E GE

Conservation physiology 20161229 1

Change in the activity of the main inhibitory receptor, GABAA, has been suggested to be a general mechanism behind the behavioural alterations reported in ocean acidification studies on fish. It has been proposed that regulatory acid-base mechanisms in response to high CO2 alter the neuronal Cl- and HCO3- gradients that are important for GABAA receptor function. Here, we report a comprehensive analysis of gene expression of GABA<s ...[more]

PMID: 28066553

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Project description:Genetic isolation and drift may imperil peripheral populations of wide-ranging species more than central ones. Therefore, information about species genetic variability and population structure is invaluable for conservation managers. The Iberian populations of three-spined stickleback lie at the southwestern periphery of the European distribution of Gasterosteus aculeatus. This teleost is a protected species in Portugal and Spain and local extinctions have been reported in both countries during the last decades. Our objectives were (i) to determine whether the Iberian populations of G. aculeatus are unique or composed of any of the major evolutionary lineages previously identified and (ii) to assess the evolutionary potential of these peripheral populations. We genotyped 478 individuals from 17 sites at 10 polymorphic microsatellite loci to evaluate the genetic variability and differentiation of the Ibero-Balearic populations. We also sequenced 1,165 bp of the mitochondrial genome in 331 of those individuals in order to complement the estimates of genetic diversity in the Ibero-Balearic region. We predicted the evolutionary potential of the different sites analysed based on the contribution of each of them to total allelic/mitochondrial diversity. An intraspecific phylogeny at European level was reconstructed using our data from the mitochondrial cytochrome b gene (755 bp) and published sequences. The so-called Transatlantic, European and Mediterranean mitochondrial lineages were found to be present in the Ibero-Balearic region. Their phylogeography suggests a history of multiple colonisations. The nuclear results show, however, a strong correlation between population structure and drainage system. The following basins should be prioritised by conservation policies in order to preserve those populations with the highest evolutionary potential: the Portuguese Vouga and Tagus as well as the Spanish Majorca and Limia. Maintenance of their connectivity, control of exotic species and monitoring of habitat properties are strongly recommended in those areas. Genetic variation alone cannot, however, ensure the persistence of these peripheral southern populations of G. aculeatus. On the one hand, the analysis of a historical sample from Eastern Spain (Penyscola) revealed no genetic erosion, which suggests a fairly sudden extinction of that population. On the other hand, the reintroduction program implemented in the Valencian Community has mostly failed despite our finding of similar level of genetic diversity between the wild source and the captive-bred released individuals.

Dataset Information

Expression of genes involved in brain GABAergic neurotransmission in three-spined stickleback exposed to near-future CO2.

Publications

Expression of genes involved in brain GABAergic neurotransmission in three-spined stickleback exposed to near-future CO<sub>2</sub>.

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