Ontology highlight
ABSTRACT:
SUBMITTER: Wang R
PROVIDER: S-EPMC5201207 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Wang Ruowen R Lu Danqing D Bai Huarong H Jin Cheng C Yan Guobei G Ye Mao M Qiu Liping L Chang Rongshan R Cui Cheng C Liang Hao H Tan Weihong W
Chemical science 20151210 3
Conjugation of DNAs to defined locations on a protein surface will offer powerful tools for positioning functional groups and molecules in biological and biomedical studies. However, tagging protein with DNA is challenging in physiological environments, which requires a bioorthogonal approach. Here we report a chemical solution to selectively conjugate DNA aptamer with a protein by protein-aptamer template (PAT)-directed reactions. Since protein-aptamer interactions are bioorthogonal, we exploit ...[more]