Ontology highlight
ABSTRACT:
SUBMITTER: van Gijtenbeek LA
PROVIDER: S-EPMC5201305 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
van Gijtenbeek Lieke A LA Robinson Andrew A van Oijen Antoine M AM Poolman Bert B Kok Jan J
PLoS genetics 20161215 12
By using fluorescence imaging, we provide a time-resolved single-cell view on coupled defects in transcription, translation, and growth during expression of heterologous membrane proteins in Lactococcus lactis. Transcripts encoding poorly produced membrane proteins accumulate in mRNA-dense bodies at the cell poles, whereas transcripts of a well-expressed homologous membrane protein show membrane-proximal localization in a translation-dependent fashion. The presence of the aberrant polar mRNA foc ...[more]