Evidence for ARGONAUTE4-DNA interactions in RNA-directed DNA methylation in plants.
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ABSTRACT:
SUBMITTER: Lahmy S
PROVIDER: S-EPMC5204349 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Publications
Evidence for ARGONAUTE4-DNA interactions in RNA-directed DNA methylation in plants.
Lahmy Sylvie S Pontier Dominique D Bies-Etheve Natacha N Laudié Michèle M Feng Suhua S Jobet Edouard E Hale Christopher J CJ Cooke Richard R Hakimi Mohamed-Ali MA Angelov Dimitar D Jacobsen Steven E SE Lagrange Thierry T
Genes & development 20161216 23
RNA polymerase V (Pol V) long noncoding RNAs (lncRNAs) have been proposed to guide ARGONAUTE4 (AGO4) to chromatin in RNA-directed DNA methylation (RdDM) in plants. Here, we provide evidence, based on laser UV-assisted zero-length cross-linking, for functionally relevant AGO4-DNA interaction at RdDM targets. We further demonstrate that Pol V lncRNAs or the act of their transcription are required to lock Pol V holoenzyme into a stable DNA-bound state that allows AGO4 recruitment via redundant glyc ...[more]
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