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Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis.

ABSTRACT: On-chip imaging of intact three-dimensional tissues within microfluidic devices is fundamentally hindered by intratissue optical scattering, which impedes their use as tissue models for high-throughput screening assays. Here, we engineered a microfluidic system that preserves and converts tissues into optically transparent structures in less than 1 d, which is 20× faster than current passive clearing approaches. Accelerated clearing was achieved because the microfluidic system enhanced the exchange of interstitial fluids by 567-fold, which increased the rate of removal of optically scattering lipid molecules from the cross-linked tissue. Our enhanced clearing process allowed us to fluorescently image and map the segregation and compartmentalization of different cells during the formation of tumor spheroids, and to track the degradation of vasculature over time within extracted murine pancreatic islets in static culture, which may have implications on the efficacy of beta-cell transplantation treatments for type 1 diabetes. We further developed an image analysis algorithm that automates the analysis of the vasculature connectivity, volume, and cellular spatial distribution of the intact tissue. Our technique allows whole tissue analysis in microfluidic systems, and has implications in the development of organ-on-a-chip systems, high-throughput drug screening devices, and in regenerative medicine.

SUBMITTER: Chen YY

PROVIDER: S-EPMC5206515 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis.

Chen Yih Yang YY Silva Pamuditha N PN Syed Abdullah Muhammad AM Sindhwani Shrey S Rocheleau Jonathan V JV Chan Warren C W WC

Proceedings of the National Academy of Sciences of the United States of America 20161212 52

On-chip imaging of intact three-dimensional tissues within microfluidic devices is fundamentally hindered by intratissue optical scattering, which impedes their use as tissue models for high-throughput screening assays. Here, we engineered a microfluidic system that preserves and converts tissues into optically transparent structures in less than 1 d, which is 20× faster than current passive clearing approaches. Accelerated clearing was achieved because the microfluidic system enhanced the excha ...[more]

PMID: 27956625

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Dataset Information

Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis.

Publications

Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis.

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