Ontology highlight
ABSTRACT:
SUBMITTER: Wang ZA
PROVIDER: S-EPMC5206893 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Angewandte Chemie (International ed. in English) 20161202 1
Using the amber suppression approach, N<sup>ϵ</sup> -(4-azidobenzoxycarbonyl)-δ,ϵ-dehydrolysine, an allysine precursor is genetically encoded in E. coli. Its genetic incorporation followed by two sequential biocompatible reactions allows convenient synthesis of proteins with site-specific lysine dimethylation. Using this approach, dimethyl-histone H3 and p53 proteins have been synthesized and used to probe functions of epigenetic enzymes including histone demethylase LSD1 and histone acetyltrans ...[more]