Alkaline Response of a Halotolerant Alkaliphilic Halomonas Strain and Functional Diversity of Its Na+(K+)/H+ Antiporters.

ABSTRACT: Halomonas sp. Y2 is a halotolerant alkaliphilic strain from Na⁺-rich pulp mill wastewater with high alkalinity (pH >11.0). Transcriptome analysis of this isolate revealed this strain may use various transport systems for pH homeostasis. In particular, the genes encoding four putative Na⁺/H⁺ antiporters were differentially expressed upon acidic or alkaline conditions. Further evidence, from heterologous expression and mutant studies, suggested that Halomonas sp. Y2 employs its Na⁺/H⁺ antiporters in a labor division way to deal with saline and alkaline environments. Ha-NhaD2 displayed robust Na⁺(Li⁺) resistance and high transport activities in Escherichia coli; a ΔHa-nhaD2 mutant exhibited growth inhibition at high

SUBMITTER: Cheng B 

PROVIDER: S-EPMC5207076 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature